Banca de DEFESA: JORGE ALVES DA SILVA NETO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : JORGE ALVES DA SILVA NETO
DATE: 29/06/2023
TIME: 08:00
LOCAL: Google Meet
TITLE:

Identification of QTLs associated with melon resistance to leaf miner fly and study of potential resistance inducers

KEY WORDS:

QTL, SSR, Liriomyza, Cucumis melo L.

PAGES: 71
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUMMARY:

One of the main methods of combating leaf miner flies is chemical control. However, the identification of resistance genes through molecular analysis can be a viable alternative in combating this pest. In addition, the search for substances capable of inducing this resistance is a promising alternative in controlling the disease. This study aimed to use microsatellite molecular markers to identify QTLs (Quantitative Trait Loci) associated with resistance and to evaluate the effect of substances capable of activating melon resistance mechanisms against leaf miner flies. For this, an F2 generation was produced from resistant (Ruthenium) and susceptible (Vedrantais) parents and evaluated with 100 microsatellite markers to identify resistance loci. In addition, eight potential inducers, four biological (autoclaved extracts of Trichoderma, Lactobacillus, Saccharomyces and Liriomyza) and four chemicals (hydrogen peroxide, sodium nitroprusside, potassium phosphite and Acibenzolar-S-methyl), were tested to evaluate the induction of melon resistance against leaf miner fly, using three concentrations and two induction methods (via seed and leaf) for each inducer used. Of the 100 SSR markers, 19 showed an excellent amplification pattern and polymorphism and, of these, 13 were considered without segregation distortions. The results identified three main markers (CMTA166, CMCT170B and CMTA134A), which respectively explain 5.73%, 4.46% and 3.32% of the resistance-related phenotypic variations. Multiple regression identified that CMTA166 and CMCT170B together explain 9.42% of the phenotypic resistance variation. As for the inducers, the most efficient treatment for the leaf miner preference rate in melon was Acibenzolar-S-methyl, both in seed application (81% inhibition) and in foliar application (69.2% inhibition). The results obtained in this study can be used in future selections of genotypes, as well as in analyzes of QTLs associated with the improvement of melon against the leaf miner fly, as well as in the use of inducing substances for the activation of resistance against this pest.

COMMITTEE MEMBERS:
Externa à Instituição - FABIANE RABELO DA COSTA BATISTA - INSA
Presidente - 1669088 - IONÁ SANTOS ARAÚJO HOLANDA
Interna - 2652583 - LINDOMAR MARIA DA SILVEIRA
Interna - 1544411 - MARCIA MICHELLE DE QUEIROZ AMBROSIO
Externo ao Programa - 1818437 - MAURICIO SEKIGUCHI DE GODOY - null