Banca de DEFESA: DEISY ALEXANDRA ROSERO ALPALA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : DEISY ALEXANDRA ROSERO ALPALA
DATE: 29/02/2024
TIME: 14:00
LOCAL: Google Meet
TITLE:

Identification of genetic polymorphism and phenotypic consequences caused by atmospheric cold plasma and 60Co gamma rays in watermelon (Citrullus lanatus (Thunberg) Matsumura & Nakai)

KEY WORDS:

Identification of genetic polymorphism and phenotypic consequences caused by atmospheric cold plasma and 60Co gamma rays in watermelon (Citrullus lanatus (Thunberg) Matsumura & Nakai)

PAGES: 97
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUMMARY:

Watermelon is a crop of economic importance in Brazil and the Northeast region. However, diversifying and improving productivity is a constant demand in production. In this sense, this study evaluated the effects of gamma rays and atmospheric cold plasma on the germination and development of watermelon (Sugar Baby variety) as well as their associations with genetic and/or phenotypic variations and their potential use. For plasma, a direct application (CAP) or through activated water (PAW) was carried out and for gamma rays, in addition to the non-irradiated control, doses of 100, 200, 300 and 400 Gy were used. Variables related to germination, structure and morphology of roots, frequencies of anomalies, morphological variables of the plant in the vegetative and flowering state up to 40 days after treatment were analyzed, as well as DNA analysis using inter sequence simple repeat (ISSR) markers. and simple sequence repeats (SSR) for plants that were treated with plasma. For gamma rays, the calculation of LD50, the fruiting state and analyzes with random amplified polymorphic fragment (RAPD) markers were additionally considered. As a result of plasma treatment, it was observed that both CAP and PAW did not generate statistically significant changes (ns) for some indices associated with germination but could improve germination potential. Furthermore, there were improvements in the effects of root length, stem length and total plant length, with these variables being changed by the less energetic conditions of the CAP and PAW processes. Plasma application promoted changes in root morphology and structure with variations in the number of secondary roots. Furthermore, anomalies were observed in the development of plants. More energetic plasma conditions generated physiological disturbances at frequencies of 24.5 to 30.5%. With treatment with PAW and CAP, a group of individuals with phenotypic characteristics different from the control was obtained. The ISSR and SSR markers evaluated did not allow the identification of changes in the DNA. The analysis of variance showed that only the variables: number of petioles, number of anthers and petal width did not undergo changes caused by gamma rays. The remaining variables evaluated also showed highly significant effects (p

COMMITTEE MEMBERS:
Externa à Instituição - YACENIA MORILLO CORONADO
Presidente - 1669088 - IONÁ SANTOS ARAÚJO HOLANDA
Externo à Instituição - JUSSIER DE OLIVEIRA VITORIANO - UFRN
Interna - 2652583 - LINDOMAR MARIA DA SILVEIRA
Externa à Instituição - SEMÍRAMES RABELO RAMALHO RAMOS - PESQUISADOR